duolink pla blocking solution Search Results


recipe duolink mounting medium  (Bio-Rad)
93
Bio-Rad recipe duolink mounting medium
Recipe Duolink Mounting Medium, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/duolink+pla+blocking+solution/Mounting+Medium/pm39906960-50-130-148
Average 93 stars, based on 1 article reviews
recipe duolink mounting medium - by Bioz Stars, 2026-10
93/100 stars
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mouse antibodies  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology mouse antibodies
Mouse Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/duolink+pla+blocking+solution/rabbit+anti-mouse+IgG-B/pm27999108-73-58-29
Average 96 stars, based on 1 article reviews
mouse antibodies - by Bioz Stars, 2026-10
96/100 stars
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duolink blocking solution  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology duolink blocking solution
( A ) Effect of DNFB (5 μM) and knockdown of MTCK or CKB <t>on</t> <t>EGFR</t> phosphorylation. Lower panels, effect of siMTCK and siCKB on expression of MTCK and CKB, respectively. ( B ) Semi-quantification of EGFR phosphorylation levels. ( C ) Effect of ATP on suppressed EGFR phosphorylation by DNFB (5 μM). ( D ) Effect of oligomycin on EGFR phosphorylation. ( E ) Effect of CyCr on creatine phosphorylation. ( F ) Effect of CyCr on EGFR phosphorylation. ( G ) Co-immunoprecipitation using anti-CKB antibody or anti-EGFR antibody to examine binding of CKB and EGFR. ( H ) <t>Duolink</t> ® proximity ligation assay. Red, proximity signal of EGFR and CKB. Blue, DAPI. Scale bar, 10 μm. Error bars: standard deviation of three independent trials. Statistical significance was calculated using a two-tailed ordinary analysis of variance. Abbreviations: DNFB: dinitrofluorobenzene; EGFR: epithelial growth factor receptor; pEGFR: phosphorylated EGFR; EGF: epithelial growth factor; CKB: creatine kinase B; MTCK: mitochondrial creatine kinase; siC: short interfering RNA (siRNA) used as control; siMTCK: siRNA for MTCK; siCKB: siRNA for CKB; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; OM: oligomycin; CyCr: cyclocreatine; pCr: phosphocreatine; IP: immunoprecipitation; CB: Coomassie blue; WCL: whole cell lysate; DAPI: 4′,6-diamidino-2-phenylindole.
Duolink Blocking Solution, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/duolink+pla+blocking+solution/EGFR+Antibody/pmc10197964-262-30-45
Average 96 stars, based on 1 article reviews
duolink blocking solution - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier

Image Search Results


( A ) Effect of DNFB (5 μM) and knockdown of MTCK or CKB on EGFR phosphorylation. Lower panels, effect of siMTCK and siCKB on expression of MTCK and CKB, respectively. ( B ) Semi-quantification of EGFR phosphorylation levels. ( C ) Effect of ATP on suppressed EGFR phosphorylation by DNFB (5 μM). ( D ) Effect of oligomycin on EGFR phosphorylation. ( E ) Effect of CyCr on creatine phosphorylation. ( F ) Effect of CyCr on EGFR phosphorylation. ( G ) Co-immunoprecipitation using anti-CKB antibody or anti-EGFR antibody to examine binding of CKB and EGFR. ( H ) Duolink ® proximity ligation assay. Red, proximity signal of EGFR and CKB. Blue, DAPI. Scale bar, 10 μm. Error bars: standard deviation of three independent trials. Statistical significance was calculated using a two-tailed ordinary analysis of variance. Abbreviations: DNFB: dinitrofluorobenzene; EGFR: epithelial growth factor receptor; pEGFR: phosphorylated EGFR; EGF: epithelial growth factor; CKB: creatine kinase B; MTCK: mitochondrial creatine kinase; siC: short interfering RNA (siRNA) used as control; siMTCK: siRNA for MTCK; siCKB: siRNA for CKB; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; OM: oligomycin; CyCr: cyclocreatine; pCr: phosphocreatine; IP: immunoprecipitation; CB: Coomassie blue; WCL: whole cell lysate; DAPI: 4′,6-diamidino-2-phenylindole.

Journal: Oncotarget

Article Title: Role of creatine shuttle in colorectal cancer cells

doi: 10.18632/oncotarget.28436

Figure Lengend Snippet: ( A ) Effect of DNFB (5 μM) and knockdown of MTCK or CKB on EGFR phosphorylation. Lower panels, effect of siMTCK and siCKB on expression of MTCK and CKB, respectively. ( B ) Semi-quantification of EGFR phosphorylation levels. ( C ) Effect of ATP on suppressed EGFR phosphorylation by DNFB (5 μM). ( D ) Effect of oligomycin on EGFR phosphorylation. ( E ) Effect of CyCr on creatine phosphorylation. ( F ) Effect of CyCr on EGFR phosphorylation. ( G ) Co-immunoprecipitation using anti-CKB antibody or anti-EGFR antibody to examine binding of CKB and EGFR. ( H ) Duolink ® proximity ligation assay. Red, proximity signal of EGFR and CKB. Blue, DAPI. Scale bar, 10 μm. Error bars: standard deviation of three independent trials. Statistical significance was calculated using a two-tailed ordinary analysis of variance. Abbreviations: DNFB: dinitrofluorobenzene; EGFR: epithelial growth factor receptor; pEGFR: phosphorylated EGFR; EGF: epithelial growth factor; CKB: creatine kinase B; MTCK: mitochondrial creatine kinase; siC: short interfering RNA (siRNA) used as control; siMTCK: siRNA for MTCK; siCKB: siRNA for CKB; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; OM: oligomycin; CyCr: cyclocreatine; pCr: phosphocreatine; IP: immunoprecipitation; CB: Coomassie blue; WCL: whole cell lysate; DAPI: 4′,6-diamidino-2-phenylindole.

Article Snippet: The following is a brief description: HT29 cells (1 × 10 5 ) were seeded and incubated for 24 h on Nunc chamber slides (Thermo Fisher), and then incubated with Duolink ® Blocking Solution at 37°C for 60 min. Anti-EGFR antibody (mouse monoclonal, 0.5 μg/mL, Santa Cruz) and anti-CKB antibody (rabbit polyclonal, 0.5 μg/mL, Boster) were incubated with the Duolink ® Antibody Diluent.

Techniques: Knockdown, Phospho-proteomics, Expressing, Immunoprecipitation, Binding Assay, Proximity Ligation Assay, Standard Deviation, Two Tailed Test, Small Interfering RNA, Control